Amino Acids in Animal Nutrition by D_Mello

By D_Mello

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Extra resources for Amino Acids in Animal Nutrition

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To check ration formulation and production. Of particular concern is the accurate and homogeneous incorporation of the supplemented amino acids as they account for a considerable proportion of the total content and have therefore a major impact on animal performance. The given good repeatability of extraction and analysis of these supplemented amino acids has led to the elaboration of a mixer test which determines concentrations and variation (CV) in typically ten samples from a mixer batch (Poole, 1991; Fontaine, 2002).

1995) tested the methodology thoroughly in food and feed samples, ruling out interference from salt in neutralized hydrolysates. The depicted chromatograms of broiler feed and maize show good separation of all amino acids in the oxidized hydrolysate. Good agreement with IEC is achieved in several samples, including for arginine and threonine. Van Wendelen and Cohen (1997) studied the separation conditions in collagen hydrolysates and cell culture media samples and designed an optimal quaternary eluent system.

The long-established, slightly cumbersome (long reaction time, distillation for removal of excess reagent in a vacuum) derivatization with PITC was made commercially accessible by Waters as the Pico-Tag method using a dedicated apparatus. The PITC amino acids being formed, which are fairly stable in an acid environment, can cyclize to phenylthiohydantoin (PTH) amino acids in an alkaline environment (secondary reaction). The first-named derivatives are usually chromatographed and detected under UV light.

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