Applied Virology Research: New Diagnostic Procedures by Christine Kurstak, A. Hossain, Edouard Kurstak (auth.),

By Christine Kurstak, A. Hossain, Edouard Kurstak (auth.), Edouard Kurstak, R. G. Marusyk, F. A. Murphy, M. H. V. Van Regenmortel (eds.)

Volume three is dedicated to the newest diagnostic know-how for virus illnesses, rather molecular methodologies.

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159-174. Williams and Wilkins, Baltimore. Rogers, M. , Thomas, P. , Schoenbaum, E. , Selwyn, P. , Grimm, K. , Bamji, M. and Schochetman, G. (1989). New Engl. J. Med. 320,1649-1654. Saiki, R. , Bugawan, T. , Horne, G. , Mullis, K. B. and Erlich, H. A. (1986). Nature 324, 163--165. Saiki, R. , Gelfand, D. , Scharf, S. J. , Horn, G. , Mullis, K. B. and Erlich, H. A. (1988). Science 239,487-491. Saiki, R. , Mullis, K. , Horn, G. , Erlich, H. A. and Arnheim, N. (1985). Science 230,1350-1354. , Walsh, R.

Detection of AZT-resistant HIV-7 using 3SR amplification followed by differential beadbased sandwich hybridization BBSH Development of effective chemotherapeutic regimens for HIV-1, based on inhibition of the HIV-1 polymerase, are likely to require the ability to monitor patients for the appearance of drug-resistant viruses (Richman, 1990). , 1990) is both time consuming and costly. , 1991). 42 D. Y. R. , 1990). Changes in four codons (codon 67, 70, 215 and 219) within the pol gene have been identified as contributing to the AZT-resistant phenotype of HIV-1 (Larder and Kemp, 1989).

However, PCR assays for HIV, particularly if amplification of the histocompatibility region is used as a control for the number of cells examined and amplification integrity of the sample, allows for simple resolution. , 1989) (see Figure 6). XIII. CARRY OVER As mentioned above, considerable care must be taken to avoid carryover of DNA from one tube to another to prevent false-positives (Higuchi and Kwok, 1989). Because amplified sequences are present in large numbers, carryover of minute quantities of amplified DNA can lead to significant false-positive problems.

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