By Christine Kurstak, A. Hossain, Edouard Kurstak (auth.), Edouard Kurstak, R. G. Marusyk, F. A. Murphy, M. H. V. Van Regenmortel (eds.)
Volume three is dedicated to the newest diagnostic know-how for virus illnesses, rather molecular methodologies.
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Extra info for Applied Virology Research: New Diagnostic Procedures
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Detection of AZT-resistant HIV-7 using 3SR amplification followed by differential beadbased sandwich hybridization BBSH Development of effective chemotherapeutic regimens for HIV-1, based on inhibition of the HIV-1 polymerase, are likely to require the ability to monitor patients for the appearance of drug-resistant viruses (Richman, 1990). , 1990) is both time consuming and costly. , 1991). 42 D. Y. R. , 1990). Changes in four codons (codon 67, 70, 215 and 219) within the pol gene have been identified as contributing to the AZT-resistant phenotype of HIV-1 (Larder and Kemp, 1989).
However, PCR assays for HIV, particularly if amplification of the histocompatibility region is used as a control for the number of cells examined and amplification integrity of the sample, allows for simple resolution. , 1989) (see Figure 6). XIII. CARRY OVER As mentioned above, considerable care must be taken to avoid carryover of DNA from one tube to another to prevent false-positives (Higuchi and Kwok, 1989). Because amplified sequences are present in large numbers, carryover of minute quantities of amplified DNA can lead to significant false-positive problems.