Fluorescence in Bio-inspired Nanotechnology: First as Probe, by Jonas Hannestad

By Jonas Hannestad

In his thesis Fluorescence in Bio-inspired Nanotechnology, Jonas Hannestad describes the evolving box of DNA nanotechnology in a lucid and simply available manner. A important topic within the thesis is how organic constructions and mechanisms represent a foundation for the layout of novel technologies. Hannestad discusses how self-assembled, nanometer-scale DNA constructs may be functionalized utilizing fluorescent labeling. particularly, he highlights how applications are in keeping with fluorescence resonance power move (FRET). one other very important contribution is the advance of a lipid monolayer platform for the step by step meeting of DNA nanoconstructs. The paintings within the thesis is predicated on five peer-reviewed papers released in high-profile journals, all of which contain significant contributions from the writer.

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Extra resources for Fluorescence in Bio-inspired Nanotechnology: First as Probe, Then as Function (Springer Theses)

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Such processes are called quenching. There are several different mechanisms behind fluorescence quenching including excited state reactions, electron transfer and molecular rearrangement. The result is an introduction of an additional decay rate, kQ, resulting in a shorter fluorescence lifetime. Fluorescence resonance energy transfer (FRET) is one type of quenching that involves the transfer of excitation energy from the quenched molecule (the donor) to the quenching molecule (the acceptor). Here, a brief overview of FRET fundamentals is provided.

7 Assembled DNA tetrahedron shown together with the constituent strands. Coloring of the strands correspond to their region in the tetrahedron. Adapted from Goodman et al. [23] 19 1 2 3 4 one step-assembly and a high structure yield of approximately 95 %. One application of the DNA tetrahedron is presented by Armitage et al. [35] who created a supramolecular multi-fluorophore assembly. This work will be discussed more in detail in the chapter focused on nanoscale photonic devices (Chap. 5). Another example comes from Alivisatos et al.

In order to describe this interaction, light has to be treated both as a wave and as a particle. The wave nature of light, described as electromagnetic radiation, consists of two components, one electric and one magnetic. These two components form two separate waves of perpendicular orientation. However, light energy is not continuous. Instead, it comes in discrete energy packages, photons, behaving as particles. 1. 1) In this expression, E is the photon energy, v is the frequency of the electromagnetic wave and h is Planck’s constant.

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